Journal: Healthcare Technology Letters

Article Title: Wearable, multimodal, vitals acquisition unit for intelligent field triage

doi: 10.1049/htl.2016.0038

Figure Lengend Snippet: Block diagram for FPGA application programmed in very high speed integrated circuit hardware description language (VHDL) with a strictly modular approach

Article Snippet: This approach emphasises the design effort on new system parts, such as multichannel auscultation, rather than reinventing existing technology, such as ECG, by also reducing design risk. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Fig. 3 caption a7 Block diagram for custom designed MCU hardware depicting modularisation and eight channel acoustic front-end FPGA: The high performance CPU is a powerful Xilinx Spartan-6 ® XC6LX9 FPGA (CSG324 package, 200 GPIOs).

Techniques: Blocking Assay

Journal: Critical reviews in biomedical engineering

Article Title: Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology

doi: 10.1615/CritRevBiomedEng.2020033450

Figure Lengend Snippet: Digital tension sensing with DNA hairpins and dsDNA. (A) The unfolding force is digital in nature as compared to more analog entropic spring probes and increases with GC content. (B) When the binding force exceeds the tension tolerance, the hairpin unfolds, allowing the previously quenched fluorophore to fluoresce. (C) Example F1/2 values, free energy, length, and GC content for hairpin probes used in the study. Reprinted with permission from Springer Nature, Copyright 2014.49

Article Snippet: As shown in the previous examples, these outputs can include changes in topography, conformation, fluorescence, or impedance. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 5: caption a7 Nanosensors made using DNA origami can be designed to independently detect the binding of analytes like nucleic acids or small molecules and use custom-designed transduction mechanisms to report binding events via changes in, for example, structural conformation (mechanical), fluorescence, and impedance (electronic).

Techniques: Binding Assay

Journal: Critical reviews in biomedical engineering

Article Title: Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology

doi: 10.1615/CritRevBiomedEng.2020033450

Figure Lengend Snippet: DNA origami sensors for measuring and applying pN and sub-pN force and nm-scale displacements. (A) Device for measuring molecular crowding forces with 100-fN force resolution. Reprinted with permission from the American Chemical Society, Copyright 2017.86 (B) Nanoscale force clamp device can apply constant tension force to central DNA duplex. (C) At the central DNA duplex, the entropic spring force is set by the length of the ssDNA and end-to-end distance set by the DNA origami fixture. Reprinted with permission from the American Association for the Advancement of Science, Copyright 2016.87 (D) Schematic of the DNA force spectrometer featuring a spring-loaded hinge with two attached nucleosomes. The torque generated by the hinge is illustrated with a torsional spring. Spheres indicate positions of fluorescent dyes (Atto647N and Atto550) that form a FRET pair. Reprinted with permission from the American Chemical Society, Copyright 2016.92 (E) Nanocaliper for probing nucleosome stability provides a sensitive measure of nucleosome disassembly and can read out transcription factor (TF) binding to its target site within the nucleosome. Reprinted with permission from the American Chemical Society, Copyright 2016.91 (F) Myosin VI tethered to a two-helix bundle (2HB) nanospring moves unidirectionally along actin against the load of the nanospring. (G) Stretch/compression dynamics of the nanospring by myosin VI at 2-mM ATP + 100-μM ADP. The kymograph shows repetitive stretching and compressing of the carboxytetramethyl-rhodamine (TAMRA)-labeled nanospring. Reprinted with permission from Springer Nature, Copyright 2016.96 (H) DNA-origami-based tension probes contain three components: a ligand-presenting domain, an origami body, and a force-sensing domain. The body is composed of a six-helix-bundle DNA origami (side and top view), in which six parallel double helices are packed on a honey-comb lattice. Upon receptor (integrin) engagement to the adhesive peptide (cRGDfk) and application of sufficient tension, the hairpin unfolds, separating the fluorophore from the AuNP and organic quencher and dequenching the dye. Reprinted with permission from American Chemical Society, Copyright 2018.99

Article Snippet: As shown in the previous examples, these outputs can include changes in topography, conformation, fluorescence, or impedance. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 5: caption a7 Nanosensors made using DNA origami can be designed to independently detect the binding of analytes like nucleic acids or small molecules and use custom-designed transduction mechanisms to report binding events via changes in, for example, structural conformation (mechanical), fluorescence, and impedance (electronic).

Techniques: Generated, Binding Assay, Labeling, Adhesive

Journal: Critical reviews in biomedical engineering

Article Title: Extending the Capabilities of Molecular Force Sensors via DNA Nanotechnology

doi: 10.1615/CritRevBiomedEng.2020033450

Figure Lengend Snippet: Nanosensors made using DNA origami can be designed to independently detect the binding of analytes like nucleic acids or small molecules and use custom-designed transduction mechanisms to report binding events via changes in, for example, structural conformation (mechanical), fluorescence, and impedance (electronic). Reprinted with permission from John Wiley and Sons, Copyright 2018.105

Article Snippet: As shown in the previous examples, these outputs can include changes in topography, conformation, fluorescence, or impedance. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 5: caption a7 Nanosensors made using DNA origami can be designed to independently detect the binding of analytes like nucleic acids or small molecules and use custom-designed transduction mechanisms to report binding events via changes in, for example, structural conformation (mechanical), fluorescence, and impedance (electronic).

Techniques: Binding Assay, Transduction, Fluorescence